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KMID : 0613820100200040607
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Journal of Life Science
2010 Volume.20 No. 4 p.607 ~ p.613
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Effect of Pine (Pinus densitlore) Needle Extracts on Synthesis of Collagen in Osteoblastic MC3T3-E1 Cells
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Jeon Min-Hee
Kim Young-Kyoung
Park Yong-Soo
Hwang Hyun-Jung
Kim Sung-Gu
Lee Sang-Hyun
Choi In-Soon
Kim Mi-Hyang
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Abstract
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Osteoporosis is a disease involving a decrease in bone mineral density and an increased risk of fractures. The MC3T3-E1 pre-osteoblastic cell line is a well-accepted model of osteogenesis in vitro. Pine needles have long been used as a traditional health-promoting medicinal food in Korea. In this study, MTT assay, the alkaline phosphatase (ALP) activity and collagen synthesis of osteoblast cells were investigated to determine the effects of pine needle extracts on cell proliferation and differentiation. Pine needle extracts were prepared using hexane, ethanol and water. The effects of the pine needle extracts were examined by comparing the results with those of commercial agents, such as proanthocyanidin. The MOT3-E1 cells exposed to proanthocyanidin showed increased proliferation in a concentration-dependent manner. The cells exposed to the hexane extract showed a similar increase in proliferation to that observed with proanthocyanidin. The hexane extract showed the highest ALP activity. Moreover, a supplement of pine needle extracts induced collagen synthesis in MC3T3-E1 cells. The pine needle extract produced the highest level of collagen synthesis at concentrations of 10~50 ㎍/㎖. These results indicate that pine needle extracts have an anabolic effect on bone by promoting osteoblastic differentiation, and may be used in the treatment of common metabolic bone diseases.
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KEYWORD
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Pine (Pinus densiflora) needle, MC3T3-E1 cell, MTT assay, alkaline phosphatase, collagen synthesis
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